Two Wheels Cycles
Two Wheels Cycles
Selling, servicing and repairing bikes for over 40 years.
01384 394653
Find out more about us down below.
Click here to view HUGE savings on top brand bikes like Giant, Trek, Whyte & more. With up to 37% off in stock and ready to roll bikes
2023 BIKES
Cycle evolution continues.
With over 40 years of experience we feel we have cherry picked the best of the worlds bikes brands to offer you.
Santa Cruz, the worlds best full suspension designs. Giant, unbeatable value for money and world leaders in carbon fibre fabrication. Cannondale, multi award winning road bikes. We The People, the very best off the peg BMX bikes. There’s not a brand of bike or component we stock that we wouldn’t use our selves, and do!
E-BIKES & Di2
Software updates, status reports, fault diagnosis, we have all the correct tools and training to take care of your e-Bikes, electronic shiftitng, ANT+ devices and cycle computers.
BRANDS
We sell everything you could possibly need for you, your bike and your ride.
Need to replace worn out parts? Desire faster wheels & smoother gears? Long for a comfier saddle & shorts, or just want something different, maybe a touch of bling? We sell everything to make all your two wheeled dreams come true from all of the worlds top component, clothing and accessories manufacturers.
CYCLE TO WORK SCHEMES
Get Your New Bike Tax-Free & Save Up To 47%.
A cycle to work scheme is a great way to purchase a bike and save up to 47%. A government scheme that lets you buy a bike through your employer and spread the cost over 12 months.
Ask in store or click here for more information.
THE WORKSHOP
Servicing, repairs & wheel building
Since the shop first opened in 1977 it has always repaired and maintained cycles. What started out with one man fixing punctures is now 5 full time mechanics dealing with everything from servicing, fork & shock servicing, hydraulic servicing, tubeless repairs and set-up, wheel building and we still do puncture repairs. Each mechanic has their own specialities so they can be assigned to a job and make sure it is done to the highest standard possible. Click here for more information and a cost guide.
Booking in, get it done when it suits you
To keep you rolling for as long as possible we offer a booking in service where you can book your bike in over the phone or email into a day of your choice. You can then drop your bike off closer to your repair day. You can of course still drop your bike in at any time to leave for repair. Bike repairs are normally turned around within the week, wheel repairs normally within a few days.
Follow us on Instagram for product photos, news and updates.
BIKE BAG HIRE
Going on holiday? Why not take the bike.
Make the job of taking your bike abroad even easier with a Polaris Cargo Bag. Available to hire for just £20 a week it will keep your ride safe from door to door.
Ask in store for more details.
ABOUT US
“STILL A PROPER BIKE SHOP”
Established in 1977 and located in the West Midlands. We sell, service & repair all forms of bikes including road, mountain, hybrid, kids and, more recently, e-Bikes. We are probably best known for high end custom build mountain bikes like Santa Cruz but we still stock and sell kids bikes, entry level hybrids, mid range mountain bikes and top end, state of the art road bikes. Each person who works here is (or has been) a keen cyclist and all have a keen interest in the industry.
We have never gone niche, never focused on just one discipline, we are everything Two Wheels… and occasionally the odd trike.
We are located on the edge of the West Midlands, half a mile from the South Staffordshire countryside and only a five minute drive to three main local cycling spots, Clent Hills, Baggeridge Country Park and Kinver Edge which includes Kingsford Downhill course. The shop has a main showroom, an upstairs showroom, four workstations, an engineering workshop, plenty of parking at the back of the shop and not forgetting the friendliest staff you’ll find anywhere.
CONTACT
Visit, call or mail us
113 Brettell Lane
Amblecote
Stourbridge
West Midlands
DY8 4BS, UK
01384 394653
sales@twowheelscycles.com
Send us a message via the form below
I am human
Opening Times
| Monday | 9:30am | 5:30pm |
| Tuesday | Closed | |
| Wednesday | 9:30am | 5:30pm |
| Thursday | 9:30am | 5:30pm |
| Friday | 9:30am | 5:30pm |
| Saturday | 9:30am | 4:45pm |
| Sunday | Closed |
FIND US
Two Wheels Cycles – 2021 Bikes
Two Wheels Cycles – 2021 Bikes
With over 40 years of experience we feel we have cherry picked the best of the worlds bikes brands to offer.
Santa Cruz, the worlds best full suspesnion designs. Giant, unbeatable value for money and world leaders in carbon fibre fabridation. Cannondale, multi award winning road bikes. We The People, the very best off the peg BMX bikes. There’s not a brand of bike or component we stock that we wouldn’t use our selves, and we do!
Giant were founded in 1972 with a mission: to create better bikes and improve the cycling experience. World leader in carbon design and fabrication and the best value for money bikes to be found anywhere.
- Mountain Bikes
- Road Bikes
- Gravel & CX Bikes
- Hybrid Bikes
- e-Bikes
- Kids Bikes
“We could get all touchy feely here and talk about how we are committed and impassioned riders, and how we love bikes so much that it somehow elevates us into something unique, but that’s kind of common fodder for these “about” tabs on bike company websites.
” ©SantaCruz
- MEGA FACTORY SALE!
- eBikes
- Full Suspension Bikes
- Hardtail Bikes
“In 1976, Trek was founded on a question: Why are no high-performance bikes made in the United States? Today, Trek gives the brightest and most passionate cycling designers and researchers the resources to question everything”. ©Trek.
- eBikes
- Mountain Bikes
- Road Bikes
- Gravel & CX Bikes
- Hybrid Bikes
- Kids Bikes
“Although we never forget our heritage, Cannondale and our dealers have come a long way since our birth in a crowded loft above a pickle factory in 1971”. ©Cannondale
- Road Bikes
- Mountain Bikes
- Gravel & CX Bikes
- e-Bikes
- Hybrid & Urban Bikes
- Kids Bikes
Liv is Giant’s women’s specific division and is the first cycling brand completly dedicated for women and just like Giant, unbeatable value for money.
- Mountain Bikes
- Road Bikes
- Gravel & CX Bikes
- Hybrid Bikes
- e-Bikes
“The true definition of freedom is that you can do what you want when you want. At Whyte that means a bike designed to work at its best whenever you need it to and whatever the weather”. ©Whyte
- Mountain Bikes
- e-Bikes
- Gravel Bikes
- Leisure & Hybrid Bikes
- Kids Bikes
Multiple World Champion Juli Furtado led the way for women in mountain biking with the original Juliana model in 1999. They have since grown into a fully fledged women’s line within the Santa Cruz family.
©Juliana Bicycles.
- Mountain Bikes
- Gravel Bikes
“Since 1995, Cervélo has engineered innovative road and triathlon/time trial bikes. Whether you are a pro in the Tour de France, are taking on your first full distance triathlon, or simply enjoy riding on the weekends, we apply engineering, ambition, and passion to make a bike that is better for you.” ©Cervélo.
- Road Bikes
- Gravel Bikes
- TT & Tri Bikes
“From GT’s namesake Gary Turner designing one of the first BMX race bikes in 1972 to our modern carbon fiber creations that redefine fast-as-hell, we’ve built a long legacy of racing and winning, yet never taking ourselves too seriously”. ©GT.
- BMX Bikes
- Mountain Bikes
- eBikes
- Road Bikes
- Hybrid Bikes
- Kids Bikes
“Every year that passes we turn a new page in the book of Wethepeople and each year we endeavour to make fundamental enhancements to our collection of complete BMX bikes in the most astounding way”.
©WeThePeople.
- BMX Bikes
Designing and shredding kickass bikes since 2012. From the same company who own We The People and Salt products, these are quality BMX bikes at a more affordable price. ©RadioBikes
- BMX Bikes
“Over time our ethos of designing bikes that are reliable and easy to use has seen hundreds of thousands of children learn the life-long skill of cycling.” ©RidgebackKids.
- Kids Balance Bikes
All prices shown are list prices
Please inquire for the best price and
different payment options including 0% finance.
Presentation on the topic: “Two-wheeled Krebs cycle”
|
Nh4 |
CO2 |
||||||||
|
malate |
|||||||||
|
Ornithine cycle |
3ATF |
ctk |
|||||||
|
aspartate |
oxaloacetate |
||||||||
|
α-ketoglutarate |
|||||||||
|
UREA |
GLU fumarate |
||||||||
|
COOH |
COOH |
COOH GLU |
α – KETOGLUE |
COOH |
|||||
|
h3 |
OVER |
NADH2 |
CHNh3 |
||||||
|
CH |
O |
CHOH |
C= |
ASAT |
|||||
|
Fumarase |
O |
Ch3 |
|||||||
|
CH |
CH |
MALAT DG |
Ch3 |
||||||
|
COOH |
2 |
COOH |
COOH |
||||||
|
COOH |
|||||||||
|
FUMARATE |
MALATE |
oxaloacetate |
ASP |
||||||
Ammoniogenesis
Ammoniogenesis occurs in the kidneys and distal tubule, the mechanism for maintaining a constant pH
|
Clearance |
cage |
||
|
distal |
|||
|
tubules |
|||
|
NaA |
CO2 |
+ |
|
|
Na+ |
|||
|
h3O |
KA |
||
|
A- |
|||
|
H |
h3CO3 |
||
|
H+ |
HCO3- |
||
|
Nh4 |
|||
|
Nh4 |
GLU |
||
|
Nh5+ |
|||
|
GLN |
|||
|
blood |
H+ + HCO3- |
|||
|
h3CO3 |
[HCO3-] |
|||
|
pH=pK+lg |
[HCO |
3 |
] |
|
|
2 |
||||
As a result,
HCO3 of the blood restores the concentration of bicarbonate, and the “retribution” for this is the excretion of protons in the urine in the form of ammonium salts.
Na+
|
Nh5A |
URINE |
Content of non-protein nitrogenous components in blood serum
|
Residual nitrogen |
14.3-28.5 mmol/l |
|
Amino acid nitrogen |
1.4-3.1 mmol/l |
|
Ammonia nitrogen |
(plasma) 7.1-21.4 µmol/l |
|
Urea |
2.5-8.3 mmol/l |
||
|
Creatine |
Men |
15.2-45.8 µmol/l |
|
|
Women |
45.8-76.2 |
µmol/l |
|
|
Creatinine |
Men |
53-106. |
|
|
Women |
44-87.2 µmol/l |
||
|
Indican |
1.18-3.18 |
µmol/l |
|
|
Uric acid |
Men |
0.12-0.45 |
mmol/l |
|
Women |
0.12-0.38 |
mmol/l |
|
1 µmol/l Features of catabolism of cyclic amino acids
Despite
to the fact that to a similar way of decay
cyclic amino acids account for
about 1%, it takes on special significance
with genetic pathology
blocked due to point mutation
activity of one of the physiological
enzymes and then accumulating
the substrate undergoes transformations,
similar to the usual decay of PVC, but with
the formation of toxic products.
All
cyclic amino acids (phene, thyr, tri,
gis) differing by radicals, basically
have alanine, which, when transaminated
gives PVC, where as a radical can
serve as indole, imidazole, phenol, benzene.
Further reactions can be depicted in
following pattern:
If
in the cell at the moment hypoxia, with
this increases the concentration of NADH + H + ,
so that the pH does not shift, restored
NAD form donates its hydrogens to homologues
PVC. This reaction is (a)
dead end – as soon as pressure O 2
returns to normal, the resulting connections
re-oxidized to the original substances,
which in mitochondria undergo
transformations similar to oxidative
decarboxylation of pyruvate. Big
part of the resulting metabolites
cyclic amino acids is toxic,
so they go to the liver for
neutralization. For these purposes, usually
glutamine is used
interacting with the final connection,
inactivates it, and the last current
blood is delivered to the kidneys excreted
in the composition of urine (see Appendix, Table 5).
Classic
example of the danger of such
products in unusual quantities
serves as a hereditary disease,
named after one of them
phenylketonuria
(oligophrenia of phenylpyruvic).
His reason
– genetic block phenylalanine-4
-hydroxylases. In these conditions
retained phenylalanine
Scheme
cyclic amino acid transformations
exposed
the above changes and in the blood
pathological products appear:
phenyl-PVC,
phenyl lactate, phenyl acetate;
easily overcome the blood-brain
barrier, they cause damage to the CNS.
IN
difference from carbohydrates and neutral
fats, during the catabolism of which are formed
only SO 2
and
H 2 O,
during the breakdown of amino acids
product other than the above substances,
is ammonia .
Given its likely toxic
effects, one should dwell on the ways
neutralization.
Ways
neutralization NH 3
Free
ammonia is toxic. First, he has
good solubility in water
accompanied by the following reaction:
Her
the product is a strong alkali, which threatens
development alkalosis .
Secondly, the TsTK is violated, since its
metabolite – α-ketoglutarate – is used
hydroxide neutralization cell
ammonium. Formed and accumulated
while glutamic acid damages
balance of amino acids, in the central nervous system its hyperproduction
provokes decarboxylation, i.e.
genesis of GABA, hence the equilibrium changes
among inhibitory and excitatory mediators.
Clinically
accumulation of ammonium (hyperammonemia)
manifested by vomiting, convulsions,
confusion and clouding of consciousness,
up to coma. Therefore, in cells
There are the following mechanisms
preventing the development of this phenomenon:
1)
Reductive amination:
2)
in many tissues (brain, liver,
kidneys, muscles) neutralization
carried out by the synthesis of glutamine
(less often asparagine) – neutral
non-toxic substance capable of
diffuse through membranes (one of
forms of transport NH 3 ).
Amidnaya
the glutamine group is often used in
amination reactions (in obtaining
glucosamine, galactosamine, nonessential
amino acids, etc.)
3)
Work of intra- and extracellular buffer
systems; with which to neutralize
NH 4 OH
to ammonium salts.
Products
neutralization of ammonia (hln, asn, salts
ammonium) are delivered by the bloodstream to
hepatocytes, where the final
inactivation of this compound:
4)
Synthesis of urea.
Urea
is the complete amide of carbonic acid
substrates in its formation are
various forms of carbon dioxide (HCO 3 –
or carboxybiotin) and ammonia (amide
glutamine group or ammonium salt):
First reaction:
leaks
in mitochondria, here carbamoyl phosphate
interacts with ornithine, which serves
kind of catalyst,
what this process is known as
ornithine cycle of urea synthesis:
formed
while citrulline, leaving the mitochondria
isomerizes to enol form:
WITH
argininosuccinate
using a specific ligase –
argininosuccinate synthase interacts
with aspartic acid:
Argininosuccinate
due to the presence of highly polarized
bonds are quite easily destroyed with
using argininosuccinate lyase:
Product
this reaction, the fumarate is usually sent
into mitochondria, where it is included in the TCA, and
arginine is hydrolyzed to urea and
ornithine.
Last
binds back to the molecule
carbamoyl phosphate, starting a new cycle.
Total:
Summing up
As a result, it should be noted that in general
urea collects all the nitrogen atoms
amino acids: its one amino group is taken
from aspartate, which can be obtained
by transamination of oxaloacetate
with any amino acid, the other
the amino group is a derivative
ammonia released from
deamination.
Two wheeled
Krebs bike – that’s what they call
researchers two cycles described by G.
Krebs because of their close relationship between
yourself (Scheme).
tricarboxylic cycle
acids, closely conjugated
with biological
oxidation and oxidative
phosphorylation
Not only
is a supplier of OA for transamination
to aspartate
but also gives CO 2 ,
HOH, ATP to run
and currents
ornithine
cycle,
one of the products
whom
and is a fumarate serving as a metabolite
TsTK.
Normal flow
urea synthesis provides:
1. final
neutralization of ammonia;
2. deliverance
organism from NH 3
and CO 2 ;
3. pH balance,
maintaining bicarbonate homeostasis
buffer system.
For control
normal course of metabolism
nitrogen-containing compounds, and mainly,
neutralization processes
ammonia in
clinic
Scheme .
Relationship between TCA and ornithine
cycle.
serves as a definition
in the blood of the next indicator
–
residual
nitrogen (Rest
W)
– i.e. estimated total content
low molecular weight nitrogenous substances,
which remain in the filtrate after
protein precipitation. In their total
about half is urea,
25% is the proportion of amino acids, values
creatine fit into 5%, urates into 4% and
etc.
Normal values
residual nitrogen fluctuate within
14-25 mmol/l, in newborns they are higher
(42–71 mmol/l).